Serial Troponin T testing based on stored samples on the clinical laboratory

Oscar Oliva Torstensson1,*, Carina Gustafsson1, Birgitta Gren1, Jan Friden1, Ola Hammarsten1

1Department of clinical chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden

Introduction: The diagnosis of myocardial infarction (MI) often rely on the change in level of the a heart damage marker Troponin T over 6 hours. Serial testing is time consuming, delay the diagnosis and appropriate treatment of MI. Often the suspected MI occurs during a hospital stay for other reasons such as following a stroke or as a complication during an operation. In these cases, serum samples are often available at the clinical lab as we have a routine to store all serum samples for 2-3 days.

Objectives: Aim: Here we have tested a routine where the serial troponin T analysis was based on previous serum samples stored at the clinical lab from the same patient.

Methods: The stability of troponin T was measured.

Results: The levels of tropionin T was stable in serum samples stored 2 days at the clinical labs with less than 5% decrease at troponin T levels of 15, 25 and 100 ng/L. A function in the laboratory module was developed to assist in calculation of relative and absolute troponin T change as well as the time between sampling. The clinical utility of this system is under evaluation

Conclusion: The automatic serial testing of troponin T based on stored serum samples is likely to save time and to aid in the care of patients with suspected MI.

Keywords: Heart, Laboratory organization